The 2001 CFL draft took place on Thursday, April 26, 2001. 48 players were chosen for Canadian Football League teams from among the eligible CIAU football players from Canadian universities, as well as Canadian players playing in the NCAA and the NAIA. Of the 48 draft selections, 26 players were drafted from Canadian Interuniversity Athletics Union institutions.
Round one
| = CFL Division All-Star | | | = CFL All-Star | | | = Hall of Famer |
Round two
| = CFL Division All-Star | | | = CFL All-Star | | | = Hall of Famer |
| Pick # | CFL team | Player | Position | School |
|---|
| 9 | Saskatchewan Roughriders | Jason French | WR | Murray State |
| 10 | Calgary Stampeders (via Toronto) | Lawrence Deck | DB | Fresno State |
| 11 | Montreal Alouettes (via BC via Winnipeg) | Pat Woodcock | WR | Syracuse |
| 12 | Hamilton Tiger-Cats | Karim Grant | LB | Acadia |
| 13 | Edmonton Eskimos | Fabian Burke | CB | Toledo |
| 14 | Calgary Stampeders | Duncan O'Mahony | K | British Columbia |
| 15 | Montreal Alouettes | Jesse Palmer | QB | Florida |
| 16 | BC Lions | Jamie Boreham | K/S | Manitoba |
Round three
| Pick # | CFL team | Player | Position | School |
|---|
| 17 | Saskatchewan Roughriders | Teddy Neptune | LB | Ottawa |
| 18 | Montreal Alouettes (via Toronto) | Phil Gibson | DL | Toledo |
| 19 | Winnipeg Blue Bombers | Ben Wearing | WR | McGill |
| 20 | Hamilton Tiger-Cats | Randy Bowles | TE | Simon Fraser |
| 21 | Edmonton Eskimos | Glenn Carson | OL | Saskatchewan |
| 22 | Calgary Stampeders | Farwan Zubedi | WR | Washington State |
| 23 | Calgary Stampeders (via Montreal) | Lukas Shaver | S | Ottawa |
| 24 | BC Lions | Scott Robinson | WR | Simon Fraser |
Round four
| = CFL Division All-Star | | | = CFL All-Star | | | = Hall of Famer |
| Pick # | CFL team | Player | Position | School |
|---|
| 25 | Montreal Alouettes (via Saskatchewan) | Shawn Gifford | OT | Charleston Southern |
| 26 | Toronto Argonauts | Kevin Eiben | S | Bucknell |
| 27 | Winnipeg Blue Bombers | Nick Tsatsaronis | RB | Memphis |
| 28 | Hamilton Tiger-Cats | Ryan Donnelly | OL | McMaster |
| 29 | Montreal Alouettes (via Edmonton) | Peter Moore | DL | Syracuse |
| 30 | Calgary Stampeders | Andrew Carter | OL | Bishop's |
| 31 | Montreal Alouettes | Steven Maheu | WR/QB | Simon Fraser |
| 32 | BC Lions | Kelly Bates | OL | Saskatchewan |
Round five
| Pick # | CFL team | Player | Position | School |
|---|
| 33 | Saskatchewan Roughriders | Mike Di Battista | WR | Ottawa |
| 34 | Toronto Argonauts | Andre Talbot | WR | Wilfrid Laurier |
| 35 | Winnipeg Blue Bombers | Howie Dryden | DB | Manitoba |
| 36 | Hamilton Tiger-Cats | Mike Waszczuk | LB | Slippery Rock |
| 37 | Edmonton Eskimos | Guillaume Petit | DL | Alberta |
| 38 | Calgary Stampeders | Jeffrey Simmer | LB | Regina |
| 39 | Toronto Argonauts (via Montreal) | Jermaine Romans | DB | Acadia |
| 40 | BC Lions | Dave Tucker | LB | Manitoba |
Round six
| Pick # | CFL team | Player | Position | School |
|---|
| 41 | Saskatchewan Roughriders | Jocelyn Frenette | G | Ottawa |
| 42 | Toronto Argonauts | Matt McKnight | S | Waterloo |
| 43 | Winnipeg Blue Bombers | Darryl Fabiani | DB | Western Ontario |
| 44 | Hamilton Tiger-Cats | Will Grant | QB | Acadia |
| 45 | Edmonton Eskimos | William Wright | DB | Bishop's |
| 46 | Calgary Stampeders | David D'Onofrio | LB | York |
| 47 | Montreal Alouettes | Phil Côté | QB | Ottawa |
| 48 | BC Lions | Eric Collings | OL | British Columbia |