Iran Football's 2nd Division 2000–01 season was played in three groups of nine teams each. The top two teams from each group advanced to the second round, and the top two teams from that round – Aboomoslem and Malavan – gained promotion to the Iran Pro League.

First round results

Group 1

PosTeamPldWDLGFGAGDPtsPromotion or relegation
1Homa169432214+831Promoted second round
2Chooka Talesh169432113+831
3Mashin Sazi168442616+1028
4Rah Ahan167452116+525
5Part Sazan Mashhad166282225−320
6Esteghlal Novin164661319−618
7Irsotter Noshahr164482318+516Relegated to 2001–02 Azadegan League
8Shahin Ahvaz164481825−716
9Shahrdari Kerman1642102131−1014

Group 2

PosTeamPldWDLGFGAGDPtsPromotion or relegation
1Payam Paykan169432313+1031Promoted second round
2Mohemmat sazi168622719+830
3Sanat Naft168532413+1129
4Nassaji Mazandaran167451915+425
5Mes Kerman166642013+724
6Saipa Zanjan164571120−917
7Petroshimi Maahshahr163761421−716Relegated to 2001–02 Azadegan League
8Fajr Sepah163581317−414
9Ararat1622121634−188

Group 3

PosTeamPldWDLGFGAGDPtsPromotion or relegation
1Aboomoslem168712713+1431Promoted second round
2Malavan169432011+931
3Esteghlal Ahvaz166821814+426
4Shemushack167452418+625
5Niroye zamini164751814+419
6Adonis164661519−418
7Bargh Tehran163581119−814Relegated to 2001–02 Azadegan League
8Bimeh Iran163491221−913
9Roghan Nabati Sari16277920−1113

Second round

PosTeamPldWDLGFGAGDPtsPromotion
1Aboomoslem1062297+220Promoted to 2001–02 Iran Pro League
2Malavan10532138+518
3Payam Paykan104241011−114
4Chooka Talesh1024486+210
5Mohemmat sazi10316810−210
5Homa10244915−610