TRNA (carboxymethyluridine34-5-O)-methyltransferase (EC , ALKBH8, ABH8, Trm9, tRNA methyltransferase 9) is an enzyme with systematic name S-adenosyl-L-methionine:tRNA (carboxymethyluridine34-5-O)-methyltransferase. This enzyme catalyses the following chemical reaction

S-adenosyl-L-methionine + carboxymethyluridine34 in tRNA ⇌ {\displaystyle \rightleftharpoons } S-adenosyl-L-homocysteine + 5-(2-methoxy-2-oxoethyl)uridine34 in tRNA

The enzyme catalyses the posttranslational modification of uridine residues at the wobble position 34 of the anticodon loop of tRNA.

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