B3 domain
In-game article clicks load inline without leaving the challenge.
The B3 DNA binding domain (DBD) is a highly conserved domain found exclusively in transcription factors (≥40 species) (Pfam ) combined with other domains (InterPro: ). It consists of 100-120 residues, includes seven beta strands and two alpha helices that form a DNA-binding pseudobarrel protein fold (SCOP); it interacts with the major groove of DNA.
B3 families
In Arabidopsis thaliana, there are three main families of transcription factors that contain B3 domain:
- ARF (Auxin Response Factors)
- ABI3 (ABscisic acid Insensitive3)
- RAV (Related to ABI3/VP1)
| protein | ARF1-[dead link] | ABI3-[dead link] | RAV1- |
|---|---|---|---|
| B3 structure derived by | molecular model | molecular model | NMR |
| B3 recognition sequence | TGTCTC | CATGCA | CACCTG |
PDB: and PDB: are only known NMR solution phase structures of the B3 DNA Binding Domain.
Related proteins
The N-terminal domain of restriction endonuclease EcoRII; the C-terminal domain of restriction endonuclease BfiI possess a similar DNA-binding pseudobarrel protein fold.
See also
- Restriction endonuclease EcoRII
- Auxin
- Abscisic acid
External links
- 2008-12-04 at the Wayback MachineKummerfeld SK, Teichmann SA (2006). . Nucleic Acids Res. 34 (Database issue): D74–81. doi:. PMC . PMID . Uses a curated set of DNA-binding domains to predict transcription factors in all completely sequenced genomes
- Classification in the "Transcription factors" table according to the database.
- 2019-10-17 at the Wayback Machine, , and 2019-10-17 at the Wayback Machine family at