BAF agar
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BAF agar or biotin-aneurin-folic acid agar is a type of agar growth medium containing peptones. It is used to grow cultures of mycorrhizal fungi. It was first described by A.J.P. Oort[Wikidata] in Nutritional requirements of Lactarius species and cultural characters in relation to taxonomy in 1981. The acidic pH (5.8-6.3) of BAF agar inhibits bacterial growth.
Typical composition
BAF agar typically contains:
- 30.0 g/L glucose
- 2.0 g/L peptone
- 0.2 g/L yeast extract
- 0.5 g/L KH2PO4
- 0.5 g/L MgSO4.7 H2O
- 10.0 mg/L FeCl3.6 H2O
- 1.0 mg/L ZnSO4.7 H2O
- 5.0 mg/L MnSO4
- 100.0 mg/L CaCl2.2 H2O
- 50.0 μg/L thiamine HCl
- 1.0 μg/L biotin
- 100.0 μg/L folic acid
- 50.0 μg/L inositol
- 15 g/L agar