Chlorophyllide-a oxygenase (EC ), chlorophyllide a oxygenase, chlorophyll-b synthase, CAO) is an enzyme with systematic name chlorophyllide-a:oxygen 7-oxidoreductase. This enzyme catalyses the following overall chemical reaction

The oxidation reaction uses molecular oxygen with nicotinamide adenine dinucleotide phosphate (NADPH) as a cofactor.

Chlorophyllide a, (R=H) is converted to chlorophyllide b, in which the methyl group show in the green box is oxidised to a formyl group.

This enzyme contains a mononuclear iron centre and is part of the biosynthetic pathway to chlorophylls.

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