o-Dianisidine
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o-Dianisidine is an organic compound with the formula [(CH3O)(H2N)C6H3]2. A colorless or white solid, it is a bifunctional compound derived via the benzidine rearrangement from o-anisidine.
o-Dianisidine is a precursor to some azo dyes by formation of the bis(diazonium) derivative, which is coupled to diverse aromatic compounds. Some commercial dyes derived from o-dianisidine include C. I. Direct Blue 1, 15, 22, 84, and 98.
o-Dianisidine is also used in assaying activity of peroxidase in lab. The general reaction of a peroxidase is as follows.
ROOR ′ + 2 e − electron donor + 2 H + → Peroxidase ROH + R ′ OH {\displaystyle {\ce {ROOR'+{\overset {electron \atop donor}{2e^{-}}}+2H+->[{\ce {Peroxidase}}]{ROH}+R'OH}}}
Where the ROOR' can be hydrogen peroxide, and the electron donor be o-dianisidine.
Safety
The manufacture and degradation of o-dianisidine, like other benzidene derivatives, has attracted regulatory attention. It is also used as a reagent in biochemistry in testing for peroxides.