The enzyme hydroxyacylglutathione hydrolase (EC 3.1.2.6, systematic name = S-(2-hydroxyacyl)glutathione hydrolase) catalyzes the following reaction:

S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate

This enzyme belongs to the family of hydrolases, specifically the class of thioester lyases. It is commonly known as glyoxalase II. It participates in pyruvate metabolism.

  • Ball JC, Vander Jagt DL (1979). "Purification of S-2-hydroxyacylglutathione hydrolase (glyoxalase II) from rat erythrocytes". Anal. Biochem. 98 (2): 472–7. doi:. PMID .